$ q_{size}$

In general spots can be expected to be unreliable if they are very small, or very large relative to the other spots in the array. This could be due to problems in the washing process of the array, or bleeding at a spot. The shape of the spot is also a measure, and if they are markedly non-circular, you can draw the same conclusion as for the size. A measurement concerning the size is given by

$\displaystyle q_{size}=\exp(-\frac{\vert A-A_0\vert}{A_0}),$    

where $ A$ is the number of pixels per spot, and $ A_0$ is the average number of pixels per spot.



Bjørn Kåre Alsberg 2006-04-06