The gprload node in SciCraft has the
following input arguments:
- Files: Names of the Agilent .txt files that are going to be analysed
- Path: String containing the path to the Agilent .txt data.
The output arguments from the node are:
- RG: RG limma object [1], containing the raw,
untransformed microarray data. The object contains the following
elements:
- R: Matrix containing the red channel foreground
intensities for each spot for each array.
- Rb: Matrix containing the red channel background
intensities for each spot for each array.
- G: Matrix containing the green channel foreground
intensities for each spot for each array.
- Gb: Matrix containing the green channel background
intensities for each spot for each array.
- weights: The spot quality weights.
- genes: Data frame containing the annotation information about
the probes, e.g gene names, Id's and spatial positions on the array.
- targets: Data frame with column ``FileName'' giving
the names of the files read.
- FilterInfo: R object containing the
information used by the FilterRG node. For example the information
concerning the amount of saturated pixels per spots. etc. which is
also contained in the .gpr files.
Bjørn Kåre Alsberg
2006-04-06