$ q_{sat}$

Sometimes the intensity of a pixel exceeds the detection range of the scanner, i.e $ 2^{16}$, this effect is called saturation. It i common to reject all spots that have more than $ 10 \% $ of its pixels saturated if mean intensity values are used, while you only reject spots with more than $ 50
\%$ saturated pixels if the median is used, since it is less sensitive to variation [5].



Bjørn Kåre Alsberg 2006-04-06