robustspline

This method implements an idea similar to print-tip loess normalisation but uses regression splines in place of the loess curves and uses empirical Bayes ideas to shrink the individual print-tip curves towards a common value. This allows the technique to introduce less noise into good quality arrays with little spatial variation while still giving good results on arrays with strong spatial variation [4].



Bjørn Kåre Alsberg 2006-04-06